ABSTRACT
A partial fragment of novel sequence (arr, adriamycin-resistant related) was previously identified using the differential display (DD)-PCR technique with adriamycin-resistant L1210 variant (L1210AdR), which shows a typical multidrug resistant (MDR) phenotypes. The present research shows the isolation of full length arr cDNA sequence. To clone the full length cDNA of arr gene, DD-PCR fragments were subjected to 5'- and 3'-Rapid Amplification of cDNA End (RACE) method. The cloned arr cDNA consisted of 770 bases and contained an open reading frame of 153 bases, encoding a protein of 51 amino acid with the molecular mass of 4 kDa by in vitro translation reactions. Northern blot analysis showed that a 770 bases transcript arr gene was overexpressed in adriamycin-resistant L1210 variant, but not in the parent suggesting that the arr gene may be involved in the adriamycin-resistant phenotypes.
Subject(s)
Mice , Amino Acid Sequence , Animals , Base Sequence , Blotting, Northern , Cloning, Molecular , DNA Primers , Doxorubicin/pharmacology , Drug Resistance, Neoplasm/genetics , Gene Expression Regulation, Neoplastic , Glycoproteins/genetics , Leukemia L1210/genetics , Leukemia L1210/drug therapy , Molecular Sequence Data , Protein BiosynthesisABSTRACT
Several derivatives of well-known natural anticancer drugs have been synthesized and will continue to be, to overcome their delivering problems, or effectiveness. Characteristics such as poor solubility, metabolic inactivation, resistance development, rapid clearance or short half-life, adverse side effects like nausea, alopecia, anorexia are common undesirable limitations to their clinical use. Drug toxicity to less sensitive tumors as those in liver or brain is another aimed goal. Recent efforts to reverse undesirable physicochemical properties of well-established natural anticancer compounds are focused. Improved antitumor action of olivacine in L(1210) leukemia with slightly soluble halides with increased life span as well as "cured" animals was observed.
Subject(s)
Humans , Antineoplastic Agents, Phytogenic/pharmacology , Ellipticines/pharmacology , Leukemia L1210/drug therapy , Antineoplastic Agents, Phytogenic/therapeutic use , Ellipticines/therapeutic use , Cell SurvivalABSTRACT
El (1-[2-cloroetil] 1-nitroso, 3 ciclohexil urea) (CCNU) es una droga antitumoral que forma parte actualmente de múltiples esquemas de poliquimioterapia en el tratamienmto de diferentes localizaciones y su obtención en el país aumentará la disponibilidad de este producto. El presente trabajo evalúa la actividad, en comparación con el producto comercial usado actualmente en la clínica oncológica y por los resultados obtenidos en los tumores experimentales utilizados, ambos productos manifiestan semejante actividad antitumoral
Subject(s)
Mice , Animals , Antineoplastic Combined Chemotherapy Protocols/pharmacology , Leukemia L1210/drug therapy , Leukemia P388/drug therapyABSTRACT
El M-tiocarbamal (morfolinditiocarbamato de sodio) es un producto sintético con el cual en estos momentos se lleva a cabo el ensayo clínico fase I. Experimentalmente se combinó con el Cis-DDP en el tratamiento de la leucemia L-1210. Los resultados obtenidos evidencian la potenciación de la acción antitumoral del Cis-DDP cuando el M-tiocarbamal se administra simultáneo o después de la administración del citostático
Subject(s)
Mice , Animals , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Cisplatin/therapeutic use , Leukemia L1210/drug therapy , Thiocarbamates/therapeutic useABSTRACT
Studies were carried out on the combination of Cimetidine (CMTD) with Cytoxan (CTX) in three murine tumors. While the combination significantly potentiated the anticancer effect of CTX in L1210 leukemia, the results with P388 leukemia were not significantly different. The results with Lewis Lung Carcinoma showed a consistent reduction in the number of metastases. However, there was no consistent concomitant prolongation in survival. The host strain, biology of the tumour and the drug used in combination with CMTD might be some of the factors responsible for the varied response.
Subject(s)
Animals , Cimetidine/administration & dosage , Cyclophosphamide/administration & dosage , Drug Therapy, Combination , Female , Leukemia L1210/drug therapy , Leukemia P388/drug therapy , Leukemia, Experimental/drug therapy , Lung Neoplasms/drug therapy , Male , Mice , Mice, Inbred DBA , Neoplasm TransplantationABSTRACT
Se estudió la actividad antitumoral del 1,3 biscloroetil nitrosourea (BCNU) sintetizado en Cuba en dos tumores transplantables de ratón, las leucemias L-1210 y P-388, así como la toxicidad de diferentes esquemas de administración del producto en ratas albinas. El producto aumentó notablemente la supervivencia de los animales tratados con respecto al control en los tumores experimentales usados y manifestó efectos tóxicos notables en el sistema hematopoyético y el hígado
Subject(s)
Rats , Animals , Female , Leukemia L1210/drug therapy , Leukemia P388/drug therapy , Nitrosourea Compounds/therapeutic use , Nitrosourea Compounds/toxicity , Neoplasm TransplantationABSTRACT
Se estudia la actividad antitumoral de extractos etanólicos de plantas que crecen en Cuba en ratones con leucemia P-388 y leucemia 1210, se realiza un tamizaje alcaloidal para las mismas especies con el empleo de reactivos precipitantes y la cromatografía en placa delgada. Las plantas pertenecen a las familias Euphorbiaceae, Rubiaceae, Leguminoseae, Clusiaceae, Moraceae, Sapotaceae, Menispermeaceae y Sterculiaceae. Contienen alcaloides las especies Aleurites trisperma Blanco, Aleurites moluccana (L) Wiel, Pithecellobium dulce (Roxb) Benth, Tamarindus indica L., Chlorophora trinctoria L. Gaud e Hyperbaena racemosa Ukb. Tienen actividad antitumoral Chamaesyce buxifolia (Lam) Small, Andira inermis (Sw) H B K y Clusia rosea Jacq
Subject(s)
Rats , Animals , Antineoplastic Agents, Phytogenic/therapeutic use , Leukemia L1210/drug therapy , Leukemia P388/drug therapy , CubaABSTRACT
The ability of Amphotericin B ('Fungizone') to alter the natural resistance of leukemia L1210 to vincristine was studied in BDF1 mice Neither Fungizone nor the "solubilizing agent" sodium deoxycholate, when used in combination with vincristine potentiated the activity of the drug against L1210. There was no change in the activity pattern of 5-fluorouracil against L1210 or vincristine against P388 lymphocytic leukemia respectively, which are sensitive to these drugs. Thus, both Fungizone and sodium deoxycholate failed to improve the activity of the drugs in either a naturally resistant or sensitive murine leukemia in vivo.
Subject(s)
Amphotericin B/administration & dosage , Animals , Antineoplastic Combined Chemotherapy Protocols , Deoxycholic Acid/administration & dosage , Drug Administration Schedule , Drug Resistance , Drug Synergism , Fluorouracil/administration & dosage , Leukemia L1210/drug therapy , Leukemia P388/drug therapy , Leukemia, Experimental/drug therapy , Mice , Mice, Inbred DBA , Neoplasm Transplantation , Vincristine/administration & dosageABSTRACT
Semecarpus anacardium Linn.f. nuts were extracted by using non-polar and polar organic solvents. Hot methanol extract and a resinous fraction, isolated from it, showed antitumour activity against P388 lymphocytic leukaemia in BDF1 mice as judged by their median survival time. Petroleum ether extract and its chromatographically isolated fraction were obtained. The latter fraction was distilled under reduced pressure to get an orange-coloured oil, (b.p. 200-20 degrees/2-3 mm). Both had antitumour activity. The orange-coloured oil, on further distillation under reduced pressure, yielded Bhilawanol. An acetyl derivative of the oil was also obtained. The latter two also had antitumour activity.